New fluorescent molecules for monitoring drug loading and release in nanoparticles
The aim of this work is to develop a fluorescent molecule for labeling different kinds of nanoparticles, particularly polymeric micelles and vesicles. The fluorophore will be covalently bound to the nanoparticles and it will switch them OFF and ON as a consequence of loading and releasing their content, respectively. To reach this aim a new fluorescent tags are being designed and developed to achieve fluorescence switching upon addition of protons.
Different strategies are currently being studied to link the fluorophore to either micelles or liposomes.
The fluorescent unit has been chosen within the family of 1,8-naphtalic-anhydride compounds and derivatised in order to:
Figure 1: scheme of derivatised fluorescent tag switchable upon protonation. R can be a protected thiolated or a good leaving group for nucleophilic substitution
P. da Silva et al. Trends Biotechnology 2001, 19, 29-34
E. Pérez-Inestrosa, U. Pischel. Org. Lett. 2011, 13, 20, 5572-5557
Y.N. Kalia, R.H. Guy. J Control Release 2004, 99, 53-62
Back to the main research page
Queen Mary, University of London, School of Biological and Chemical Sciences, Mile End Road, London E1 4NS, Tel: +44 (0) 20 7882 3268
© 2010 All rights reserved to Marina Resmini Research Group